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In addition, Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), antibiotic–antimycotic, l-glutamine (GlutaMAX), sodium pyruvate, trypsin-ethylene diamine tetra-acetic acid, phosphate-buffered saline, Dulbecco’s phosphate-buffered saline, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Thermo Fisher Scientific, Waltham, MA, USA) were purchased