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The main rationale for cycling rather than continuous use involves conservative safety practices given limited long-term human data, cost management, and theoretical concerns about receptor desensitization with extended exposure

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Of the 1050 dynamically regulated phosphorylation sites previously identified by SILAC-based quantitation of five EGF stimulation time points of HeLa cells 2 (Supplementary Table 6), we covered 597 of them in our label-free DIA analysis and 504 of these sites in the DDA analysis, respectively