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The oxidation of conjugated double bonds in β-carotene at random sites—along with eccentric cleavage at double bonds other than the central one (which can also be catalyzed by β-carotene 9′10′-dioxygenase 2)—forms aldehydes, retinal, and β-apocarotenals of varying chain lengths (Figure 4)

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So, you would have already purified proteins corresponding to the full-length and each individual domain of the interacting protein, and now you do a pull-down to see which ones bind