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A growing body of evidence is highlighting the pivotal role played by adventitial cells in blood vessel remodeling and the maintenance of vascular homeostasis ( 5.1 Role of vascular endothelial cell senescence in vascular calcification VECs adeptly sense a myriad of stimuli coursing through the bloodstream and promptly respond, upholding vascular equilibrium via autocrine or paracrine mechanisms
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Cell lysates were pelleted by centrifugation at 30,000 × g for 30 min and resuspended in Earle’s Balanced Salt Solution with Ca 2+ (pH 7.4)
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